This assay has been adapted to the sebia capillary electrophoresis instruments capillarys 2 and minicap in order to provide complete automation. Keren, md, deborah hedstrom, mtascp, ronald gulbranson, mtascp, chingnan ou, phd, richard bak, phd, comparison of sebia capillarys capillary electrophoresis with the primus highpressure liquid chromatography in the evaluation of hemoglobinopathies, american journal of clinical pathology, volume, issue 5, november 2008, pages. The sebia capillarys 2 neonat fast system is now available as part of perkinelmer s total newborn screening solution. It produces a high performance level and focuses on stringent requirements for clinical diagnostic laboratories. Sep 2015 sebia focus capillarys 2 flex piercing n15. There was a good concordance between the results of capillary electrophoresis and hplc. The analytical precision for the determination of hba2 and hbf percentages was. Capillarys 2 flex piercing is an instrument utilizing capillary electrophoresis developed to be versatile while providing a clearcut and precise protein separation with high throughput.
Evaluation of the sebia capillarys 2 flex piercing for the. The capillarys neonat hb kit is designed for the separation of the normal hemoglobins f and a in blood samples from human newborns, and for the detection of the major hemoglobin vanants s, c, e, d and barts, by electrophoresis in alkaline buffer ph 9. In a normal spcze, there are four fractions seen on the graph obtained from the electrophoretogram fig. Diluent vzorku priprava diluent je pripraven k pouziti. Capillary electrophoresis system capillarys 2 flex piercing sebia. Pdf sebia capillarys 2 versus the helena biosciences v8. Evaluation of hemoglobin a1c measurement by capillarys 2. The predicate device used in this submission is sebias capillarys immunotyping using the capillarys 2, k500. Analytical evaluation of the capillarys 2 flex piercing.
We report two cases of haemoglobin new york hbny discovered through hba 1c analysis using capillary electrophoresis. It features whole blood analysis, with no preanalytical preparation. The goal of this study was to evaluate the integration of the new generation of automated capillary electrophoresis instrument, the capillarys 2 flex piercing sebia, in the daily practice of a specialized pathology laboratory. Capillary electrophoresis system capillarys 2 sebia. After each use, the buffer must imperatively be stored refrigerated between 2.
Sebias innovative capillary electrophoresis system is designed to optimize and completely automate electrophoresis testing. Figure 1 from a comparative evaluation of the analytical. Yang z1, harrison k, park ya, chaffin ch, thigpen b, easley pl, smith ja, robinson ca, lorenz rg, hardy rw. Capillarys hb a1c using the capillarys 2 flexpiercing. Capillarys hemoglobine using the capillarys 2 flexpiercing instrument intended use the capillarys hemoglobine kit is designed for the separation of the normal hemoglobins a, a2 and f in human blood samples, and for the. Given the length of this study and the number of specimens involved, multiple lots of kitsreagents were used. For optimal use of the normal hb a2 control with the capillarys 2, capillarys 2 flex. The capillarys 2 and capillarys 2 flex piercing instruments performs all.
The walkaway nature of the agarose gel electrophoresis systems provides substantial labor and cost savings to clinical laboratories. Dec 27, 20 our institution replaced spage hydrasys, sebia, norcross, ga as a screening tool with serum protein capillary zone electrophoresis spcze. To date, we were using the hplc method to perform the hba1c measurement. Capillary electrophoresis ce technology is utilized to separate, detect, and quantify normal hemoglobins and hemoglobin variants. For rinsing capillaries in automated instrument capillarys 2 flexpiercing, sebia, for capillary electrophoresis. Sebia capillarys 2 manual indy any swing goes pdf book. Info free download books sebia capillarys 2 manual printable 2019 everyone knows that reading sebia capillarys 2 manual printable 2019 is beneficial, because. Sebia electrophoresis products test menu hemoglobin. Capillarys 2 and capillarys 2 flex piercing instruments, for capillary electrophoresis. Since hba1c became a diagnostic criterion for type 2 diabetes, assays measuring this analyte were required to be analytically stronger. We evaluated the performance of the capillarys 2 sebia, norcross, ga capillary electrophoresis system for detection and identification of monoclonal proteins in serum samples. A comparative evaluation of the analytical performances of capillarys 2 flex piercing, tosoh hlc723 g8, premier hb9210, and roche cobas c501 tinaquant gen 2 analyzers for hba1c determination. To the best of our knowledge, this is the first detailed analysis of the sebia capillarys 2 flex piercing system performed in the us.
Hb ce was performed on a capillarys 2 instrument sebia, lisses, france using the capillarys hemoglobine kit, following the manufacturers instructions. Pdf evaluation of the sebia capillarys 2 flex piercing for the. Jaisson s, leroy n, meurice j, guillard e, gillery p. We evaluated the sebia capillarys 2 flex piercing cap 2fp. This programmable, multitasking densitometer speeds sample processing by allowing the operator to simultaneously perform various functions. A comparative evaluation of the analytical performances of. It is essential that hba 1c assays are capable of identifying haemoglobinopathies. It is used in conjunction with the capillarys proteine 6 kit, sebia, designed for proteins separation into 6 major fractions in alkaline buffer ph 9. Sebia, lisses, france for measurement of hemoglobin a1c hba1c on venous and capillary blood samples. These samples were evaluated in three other common hba 1c methods. Performance of the sebia capillarys 2 for detection and. Chromatograms of the same whole blood sample hbd iran analyzed by variant ii and g8 hplc methods a and. Sebia capillarys 2 versus the helena biosciences v8 capillary.
November 1, 2019 sebia karen anderson director of regulatory. Sebia capillarys 2 manual printable 2019 read online at baseballaccessories. The applications of capillary electrophoresis in medical diagnostics have expanded considerably since the early 2000s. Sebia capillarys 2 neonat fast trivitron healthcare. Sebia continuously reinvests in research and development of new products to help automate workflow and increase productivity in the clinical laboratory. Sebia, lisses, france for measurement of hemoglobin a1c. Hb electrophoresls procedure used with the capillarys 2 system, and,for the quantitative quality control for detection of the human hemoglobins a, f and a2 with the sebia electrophoresis procedures.
Materials and methods agarose gel electrophoresis protein electrophoresis was performed using the semiau. Sebia capillarys 2 versus the helena biosciences v8 capillary electrophoresis analyser for carbohydratedeficient transferrin measurement. Kolonne kolonnen, som anvendes pa tosoh hlc723g8, er en tskgel glyco hsi fra tosoh tokyo, japan. Heylen o1, van neyghem s1, exterbille s1, wehlou c1, gorus f1, weets i 2. Performances of capillary electrophoresis and hplc. The comparison was based on the correlation between our current capillary electrophoresis instruments capillarys 2 flex piercing, sebia, france and the capillarys 3 tera. Sebia capillarys 2 manual if searching for a ebook sebia capillarys 2 manual in pdf format, then you have come on to the correct site.
Identification of haemoglobin new york by haemoglobin a. Comparison of sebia capillarys capillary electrophoresis with. It has a comprehesive test menu, which includes the tsting of protein, immunotyping protein, immunotyping, urine protection, urine immunotyping, hemoglobin, hba1c and cdt. Sebia electrophoresis products hydrasys hydrasys 2. Pdf evaluation of sebia capillarys 2 flex piercing for the. Normal serum proteins separate into six major fractions. Obsahuje pridavne latky nezbytne nutne pro optimalni provedeni a efektivni separaci izoforem transferinu, v koncentracich pro cloveka neskodnych.
To evaluate the analytical performance of a new capillary electrophoresis instrument, the capillarys 2 flex piercing sebia, france, allowing the separation and quantitative estimation of the different haemoglobin fractions from whole blood, in capped primary tube. Each vial of the stock capillarys minicap wash solution sebia, pn 2052, 2 vials, 75 ml should be diluted up to 750 ml with distilled or deionized water. This study compares two automated capillary electrophoresis ce systems, the capillarys 2 sebia, surrey, uk and v8 helena biosciences, tyne and wear, uk for the measurement of carbohydratedeficient transferrin cdt. Capillarys 2 flex piercing is an instrument utilizing capillary electrophoresis developed to be versatile while providing a clearcut and. Exclusive capillary electrophoresis technology for high resolution separation and complete walkaway automation, from hemolysate preparation to final colorcoded results. Kalibratorer og kontroller til kalibrering af capillarys 2 flexpiercing anvendes hba1c capillary calibrator 1 og 2 fra sebia lisses, frankrig, og af kontroller anvendes hba1c capillary control 1 og 2 fra sebia lisses, frankrig. Used sebia capillarys 2 flex piercing lab general for. Perkinelmer, the global market leader in neonatal screening, and sebia a forerunner in capillary electrophoresis, have joined forces to create the new standard in newborn screening of hemoglobin disorders. Evaluation of sebia capillarys 2 flex piercing for the measurement of hba1c and structural variants of hemoglobin detection. Hydragel hemoglobine used with the hydrasys system, capillarys hemoglobine used with the capillarys. It has eight narrow capillary tubes which fill with electrolytes and buffers function. Sebia hydrasys 2 scan somagen the hyrys 2 densitometer is reflective of sebias expertise in the development and design of imaging systems and densitometers utilized in the electrophoresis field. Routine use of the sebia hba1c assay with the capillarys 2 flex piercing at the analysis lefaurepetit laboratory in epinal.
In the blandaltman plots the solid lines show the mean difference, while the dotted lines show the mean difference 1. This assay has been adapted to the sebia capillary electrophoresis instruments capillarys 2 and minicap in order to. For optimal use of the normal hb a2 control with the capillarys 2, capillarys 2 flexpiercing and minicap instruments, it is recommended to split the control into aliquots in microtubes before freezing see paragraph storage and stability. Quantifytotalproteinconcentrationintheurinesampletobeanalyzed. Application of capillarys 2 flex piercing hba1c measurement system for alpha and betathalassemia screening. Haemoglobinopathies may interfere with the haemoglobin a 1c hba 1c measurement, leading to incorrect diagnosis and inappropriate treatment. Evaluation of hemoglobin a 1c measurement by capillarys 2 electrophoresis for detection of abnormal glucose tolerance in african. Pdf a comparative evaluation of the analytical performances. It is a fully automated, innovative multiparameter system. This technological breakthrough was then deployed on minicap flexpiercing 20 and more recently on capillarys 3 tera 2014. The analytical precision for the determination of hba2 and hbf percentages was satisfactory and within the. Sebia capillarys 2 neonat fast the best choice in newborn hemoglobin screening.
Capillarys 2 flex piercing the reference in routine capillary electrophoresis for medical laboratories. Capillarys 3 tera 12 capillaries capillarys 2 8 capillaries minicap 2 capillaries each sample is diluted in a dilution buffer and the capillaries are filled with the separation buffer. Performance of the sebia capillarys 2 for detection and immunotyping of serum monoclonal paraproteins. List of ngsp certified laboratories updated 420, listed. Weykamp c, waeninkwieggers h, kemna e, siebelder c. Analytical evaluation of the capillarys 2 flex piercing for. We presented the utter edition of this ebook in pdf, doc, epub, djvu, txt formats. Performance of the sebia capillarys 2 flex piercing. Download sebia capillarys 2 manual indy any swing goes book pdf free download link or read online here in pdf. Capillarys cdt 2 kit je urcen k separaci izoforem transferinu v alkalickem pufru o ph 8. Although precision of sebia capillarys 2 flex piercing was previously evaluated with standard methods and material, we wanted to document the longterm performance of this analyzer in two different centers.
Capillary zone electrophoresis for haemoglobinopathy diagnosis. Evaluation of the longterm imprecision of the capillarys 2. We report two cases of haemoglobin new york hbny identified by hba 1c analysis using capillary electrophoresis capillarys 2 flex piercing c2fp, sebia during an epidemiology study. Precision of capillarys 2 for the detection of hemoglobin. Capillarys proteine 6 207 intended use the capillarys proteine 6 kit is designed for the separation of human serum and urine proteins in alkaline buffer ph 9. Identification of haemoglobin new york by haemoglobin a1c. Sebia s innovative capillary electrophoresis system is designed to optimize and completely automate electrophoresis testing. For over 40 years, sebia has been a global leader in innovative electrophoresis technology. Capillarys 2, sebia because of the aforementioned advantages. This instrument is versatile and comes standard with capped tubes for blood, urine and serum. The capillarys 2 utilizes liquid flow electrophoresis for applications in the clinical diagnostic setting. Original papers a comparative evaluation of the analytical performances of capillarys 2 flex piercing, tosoh hlc723 g8, premier hb9210, and roche cobas c501 tinaquant gen 2 analyzers for hba1c determination xiaobin wu, yan chao, zemin wan, yunxiu wang, yan ma, peifeng ke, xinzhong wu, jianhua xu, junhua zhuang, xianzhang huang department of laboratory.
The hydrasys and hydrasys 2 are compact systems that carry out all phases of electrophoresis from sample application to migration to incubation to staining, destaining and drying. We report our evaluation of automated ce, using the capillarys 2 flex piercing instrument, as a routine diagnostic method for the detection of variant haemoglobins and the diagnosis of. List of ngsp certified laboratories updated 420, listed by date certified 2 laboratory methods certification type date certified northwest lipid metabolism and diabetes research laboratories, seattle, wa tosoh g8 level i laboratory february, 2020 fundacion valle del lili, cali, colombia sebia capillarys 2 flex piercing. Pdf backgroundaim glycated hemoglobin hba1c is considered the gold standard for assessing the compensation and treatment of diabetes. The capillarys neonat hb kit is designed for laboratory use. In this study, we compared the performance of the capillarys 2 system with that of the hydrasys system sebia for the detection of paraproteins and immunotyping. All books are in clear copy here, and all files are secure so dont worry about it.640 1220 354 79 403 1179 964 536 1428 413 22 463 1060 812 879 315 1488 262 535 761 111 737 1110 1450 960 98 441 96 1109 1299 279